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Old 01/15/2018, 09:25 AM   #401
taricha
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Join Date: Mar 2010
Location: NE Miss
Posts: 608
Grrrr. I contaminated my culture I just got of parvocalanus pods with some tisbe (or similar).
I have strainers of 10, 25, 40, 150 & 250 microns.
I'm feeding live T-iso.
Ideas for isolating parvocalanus?


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Old 07/09/2018, 05:40 PM   #402
purpletang123
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Hello, thank you for your contribution to the community. I tried multiple times to access the links and none of them work... I keep getting the (404 Not Found). Please check the issue and thank you once again.


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Old 09/09/2018, 11:58 PM   #403
Hodad2
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Join Date: Aug 2017
Posts: 53
Quote:
Originally Posted by taricha View Post
Grrrr. I contaminated my culture I just got of parvocalanus pods with some tisbe (or similar).
I have strainers of 10, 25, 40, 150 & 250 microns.
I'm feeding live T-iso.
Ideas for isolating parvocalanus?


I think youíll have to start over. Thereís no way to be able to seperate by species using sieves. Only different sizes of animal. I always do that too. I just let what species of pod just take over. Itís near to impossible to maintain isolated cultures, especially at home. Make sure that you use good isolation techniques like keep cultures away from each other, plastic curtains, etc. I raise pods and rotifers for breeding fish and shrimp. Most of the time it doesnít really matter if itís contaminated.


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Old 09/10/2018, 12:06 AM   #404
Hodad2
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Join Date: Aug 2017
Posts: 53
If anyone need sieves or specifically sized aquaculture mesh for your culture projects I have a lot of sizes. Just send me a pm if u need anything.


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Old 09/10/2018, 12:11 AM   #405
Hodad2
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Posts: 53
Quote:
Originally Posted by taricha View Post
I'll post this here because I didn't know why cultures were failing until it happened.

Some of the components used in growing phyto go bad over time.
My f/2 mix from Florida aqua farms grew me many many great liters of T-iso & nanno over 6-8 months. But then in the last month, I couldn't get any culture up and running.
The f/2 mix is susceptible to bacterial degrading over long time. Some products explicitly list expiration after months for this reason.

Another thing that I didn't know is susceptible to bacterial degrading is the sodium thiosulfate that neutralizes the bleach after sterilization.

On another note, every batch I'd ever cultured up from Phycopure Copepod Blend has produced T-iso and grown parvocalanus pods very well.
Except the last batch, which grew.....



...rhodomonas!


Iím having the same problem with f/2 from Florida Aquafarms. Last batch did not grow at all. They also do not carry much more than nannochloropsis disks. Iím wondering if theyíre going out of business. That would be a shame. Are you getting contaminated source cultures from them ?


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